Peptide Discovery Synthesis
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Home | Services & Solutions | Peptide | Discovery Synthesis | Peptide
Accelerate Peptide Synthesis with Our Strong Chemistry Capability
WuXi TIDES peptide discovery platform provides library screening and cusom synthesis to address your needs and offer flexible options for various scales, purity levels, modifications requirement.
In 2023, we provided discovery peptide services for 350+ customers globally and delivered 30,000+ peptides from mg to kg scale.
We support the following peptide modalities, including but not limited to:
- Long Linear Peptide
- Cyclic Peptide
- Modified Peptide
- PEGylated Peptide
- Peptidomimetic
Service Highlights
Rich Experience
linear peptide up to 200 AAs, macrocyclic peptide, modified peptide and peptide based complex conjugates
Broad Scale
scale from mg to kg, purity up to 99.9%
High Success
Rate
over 99% on time delivery
Comprehensive Analysis Support
default purity/MS profile and additional services
High-Throughput Peptide Synthesis
up to 1,000 peptides synthesis in parallel
Quick Turnaround
Time
2 weeks turnaround time for most peptides under 30 AA at mg scale
Deliverables
Form
- Lyophilized powder
- Stock solution
Certificate of Analysis
- HPLC/UPLC and LCMS
- ID, barcode of vials
- Sequence/structure
- Quantity, purity
- Storage condition, etc.
Packaging and Logistics
- Nitrogen packaging available
- Ambient logistics worldwide
- With icebag
- Dry ice logistics with optional thermal control
Note: Additional Services (TFA removal, analytical HPLC, etc.) are available at additional cost
Library Synthesis
| Item | <10 AAs | 10-20 AAs | 20-30 AAs |
|---|---|---|---|
| Number of Peptides | 48 peptides in parallel synthesis | ||
| Delivery Time | <8 days | <12 days | <20 days |
|
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Peptide Companion Prediction-assisted assessment can be applied prior to automated synthesis.
▸ Insoluble (and/or expensive) AAs (building blocks) are not recommended for automated synthesis. ▸ The microwave-assisted synthesizer excels in facilitating difficult peptide synthesis. ▸ The combination of automated and manual synthesis is employed to expedite the process of long peptide synthesis and enhance the success rate. |
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With ~20 various high throughput synthesizers, we will support peptide library synthesis from 10 µmol – 1.0 mmol. We can synthesize peptide libraries up to 800 peptides in parallel with high success rate up to 96%. Our strong analytical support ensures high quality products with the capability to reach up to 99.9% purity.
Our team has in-depth experience with 50,000+ linear peptides and 100,000 cyclic peptides and modifications in the past years.
Automatic Peptide Synthesizer
We have 20+ high throughtput synthesizers, supporting the scale ranging from 10 µmol – 1.0 mmol.
CEM Liberty Blue
- Sequential synthesis
- 12-channel
- 25 µmol - 0.2 mmol
Biotage Syro II
- Parallel synthesis
- 36/48/96/192 channel
- 10 µmol - 0.2 mmol
CSBio Synthesizer
- Parallel synthesis
- 3-channel
- 0.2-0.5 mmol
Custom Synthesis
Linear Peptide Synthesis
Approach:
- Fmoc Solid-Phase Peptide Synthesis (SPPS)
- Fragment Coupling
- Ligation
Experience Highlight
- Length up to 250 AAs
- More than 50,000 linear peptides and modifications delivered
- More than 10,000 complex peptides delivered, including PNAs, peptoids, branched peptides, dendrimers, peptidomimetic peptides
Cyclic Peptide Synthesis
Cyclization is a prevalent strategy employed to enhance the pharmacological properties of a peptide from its linear precursor. These enhancements may include improved affinity and specificity, proteolytic stability, and/or solubility.
Our teams possess a wealth of experience in cyclic peptide synthesis, successfully delivered over 100,000 cyclic peptides.
We utilize a variety of cyclization strategies such as single/double/triple disulfide, click cyclization, lactone, lactam, and RCM. The possible cyclization positions include head-to-tail, head-to-sidechain, sidechain-to-sidechain, and sidechain-to-tail.
Head to Tail
Head to Sidechain
Sidechain to Sidechain
Sidechain to Tail
Experience in:
- Disulfide Peptide (single/double/triple disulfide)
- Thioether Cyclization
- Lactam Cyclization
- Lactone Cyclization
- Click Peptide
- RCM Cyclization
- Bicyclic Peptide
Bicyclo-disulfide Bridge
(three pairs of S-S bond formation achieved as well)
RCM Monocyclic Peptide
Bicyclic Peptide
Click Peptide
Thioether Cyclization
Modified Peptide Synthesis
We offer a comprehensive range of specialized modifications to meet your specific requirements. These include N-terminal modifications, C-terminal modifications, dye/fluorescent labeling, biotin-labeling, PEGylation, post-translational modifications, DOTA/NOTA labeling, and isotope labeling.
Beyond sidechain and backbone modifications, we are also capable of incorporating unnatural amino acids into the sequences. For more information on our catalog of unnatural amino acid products, please visit here.
Modified Peptide Synthesis
- H (free amine)
- Acetylation
- Fmoc
- CBZ
- Bz
- Pyr
- Biotinylation
- Lipid tail
- Dye and fluorescent labeling
- -OH (free)
- Ester (-Ome)
- -NH2
- -NH-Me
- Active ester (NHS; TFP; PFP)
- Dye and fluorescent (by conjugation)
- Biotin；
- FITC；
- 5-FAM (5,6-FAM)；
- Dansyl-
- TAMTA-
- MCA-
- Rhodamine
- Acetylation (Lys)
- Methylation (Arg)
- Phosphorylation (Ser, Thr, Tyr)
- Lipidation
- Glycosylation
- SUMOylation
- Ubiquitionation
- 1H – D
- 12C – 13C
- 14N – 15N
- 13C, 15N – Lys
- 13C, 15N – Arg
- 13C, 15N – Ala
- 13C, 15N – Ile
- 13C, 15N – Leu
- 13C, 15N – Pro
- 13C, 15N – Phe
- 13C, 15N – Val
- N-linked glycopeptide synthesis
- O-linked glycopeptide synthesis
- C-linked glycopeptide synthesis
- S-linked glycopeptide synthesis
Advanced Peptide Synthesis
We support the following advanced peptides.
- Ligated Peptide
- Peptidomimetics
- Peptoid
- PNA
- Branched Peptides
- Dendrimers, and more
Various Reactions on Solid Support
- Suzuki Coupling
- Sonogashira Coupling
- Heck Reaction
- Reductive Amination
- Ugi Reaction
- Wittig Reaction
- Hoffman Degradation
- Curtis Rearrangement
- Reduction/Oxidation, and more
Related Resources
WuXi TIDES Brochure: CRDMO Platform for Oligonucleotide, Peptide and Complex Conjugate
Peptide CRDMO Chemistry Platform
