Monomer & Ligand
- Select
-
- Select
-
- Select
-
- Select
-
Home | Services & Solutions | Oligonucleotide | Development & Manufacturing | API | Monomer & Ligand
Amidite Production
Our team has extensive experience with 1,000+ customized amidite molecules with most challenging structures, to support your oligonucleotide projects from discovery to development and commercial stage, including:
- Sugar backbone (LNA, cEt, UNA, GNA, TNA amidite, etc.)
- Phosphorus backbone (PACE, PS2, etc.)
- 2'/3'/5' modified amidite
- Base modified amidite (tRNA base, deaza base)
We provide amidite development and manufacturing services and have dedicated plants for amidite production. Our capacity is more than 10 metric tons/year.
Sample amidite molecules
RNAi-Amidite
(2′-F, OMe Moe, OTBS, etc.)
LNA Amidite
TNA Amidite
Vpm-POM
DNA C(Bz) Amidite
PACE Amidite
Thio-Amidite
PMO Monomer
GalNAc Production
Our teams have extensive experience in the production of various types of GalNAc molecules, including Mono-GalNAc, Tri-GalNAc, Tetra-GalNAc, GalNAc amidite, GalNAc PFP ester, GalNAc N3, GalNAc-PEG conjugate, etc.
We provide GalNAc custom synthesis, process development and manufacturing supporting from discovery phase all the way to commercial launch with batch scales ranging from milligrams to over 50 kilograms.
With our oligonucleotide teams at the same site in Changzhou, China, we provide a streamlined GalNAc-oligonucleotide conjugate development and manufacturing service. With various GalNAc building blocks and CPG/PS conjugate resins in stock, we can further accelerate your GalNAc-oligonucleotide projects.
Overcome GalNAc synthesis challenges
There are many synthetic challenges for GalNAc because most of the intermediates are oily or foamy solids. The synthesis involves many columns and prep-HPLC purification steps, resulting scale-up difficulties and low yields. Analytical method for GalNAc synthesis is also challenging due to low UV response, complex impurity profile and poor stability.
Original Process
Oil Intermediates
Modified Process
Solid Intermediates
Enabled by strong chemistry platform, WuXi TIDES developed convergent routes through telescoping processes without column purification steps. The isolated intermediates were stable solids. We applied the precipitation process and/or spray drying process to reduce the needs for the columns and prep-HPLC steps. Our analytical teams identified many GalNAc-related impurities to support method development and release testing.
